All Optical
All-optical control of neuronal circuits by optogenetics and wave front shaping
Manipulation of neuronal circuits enabling the control of each neuron independently, with single-spike precision and single-cell resolution is the next challenge to be faced in optogenetics. This means moving from whole-region optogenetics to “circuit optogenetics”. As a solution to this challenge, we propose an interdisciplinary approach combining optogenetics with wave front shaping, holographic illumination, compressed sensing and opsin engineering. We will apply this cutting-edge technology for studying neuronal circuits in optically and physiologically diverse neural systems including the mammalian cortex and the zebrafish larva.
Publications
- O. Shemesh, D. Tanese, V. Zampini, L. Changyang, P. Kiryln, E. Ronzitti, E. Papagiakoumou, E.S. Boyden, V. Emiliani [2017]. Temporally precise single-cell resolution optogenetics. Nature Neuroscience 20, 1796–1806.
