The Emiliani lab (IDV, Paris, France) develops advanced optical methods for the investigation of visual circuits in vitro and in vivo.
The “Wave front engineering microscopy” group comprises 20 people with complementary expertise in non-linear optics and neurophysiology. The team has pioneered the use of wave-front engineering for neuroscience. We destablished novel techniques for non-linear efficient photoactivation of caged compounds and optogenetics molecules: computer generated holography, generalized phase contrast & temporal focusing.We now focus on the use of these innovative approaches to investigate the functional connectivity and signal processing in the visual system.
Publication 1: Nicolò Accanto email@example.com want, we loose some light, but we can optimize this. antion he sample plane, so it decides which areas , Clément Molinier, Dimitrii Tanese, Emiliano Ronzitti, Zachary L. Newman, Claire Wyart, Ehud Isacoff, Eirini Papagiakoumou and Valentina Emiliani Multiplexed temporally focused light shaping for high-resolution multi-cell targeting. Optica 5, 1478-1491 (2018).
Publication 2: Alexis Picot, Soledad Dominguez, Chang Liu, I-Wen Chen, Dimitrii Tanese, Emiliano Ronzitti, Pascal Berto, Eirini Papagiakoumou, Dan Oron, Gilles Tessier, Benoît C. Forget, and Valentina Emiliani, Temperature rise under two-photon optogenetics brain stimulation, Cell Report 24, 1243–1253 (2018).
Publication 3: O. Shemesh, D. Tanese, V. Zampini, L. Changyang, P. Kiryln, E. Ronzitti, E. Papagiakoumou, E.S. Boyden, V. Emiliani, Temporally precise single-cell resolution optogenetics, Nature Neuroscience 20, 1796–1806 (2017).