All Optical

All Optical
All-optical control of neuronal circuits by optogenetics and wave front shaping

Manipulation of neuronal circuits enabling the control of each neuron independently, with single-spike precision and single-cell resolution is the next challenge to be faced in optogenetics. This means moving from whole-region optogenetics to “circuit optogenetics”. As a solution to this challenge, we propose an interdisciplinary approach combining optogenetics with wave front shaping, holographic illumination, compressed sensing and opsin engineering. We will apply this cutting-edge technology for studying neuronal circuits in optically and physiologically diverse neural systems including the mammalian cortex and the zebrafish larva.


  • Publication 1: C. Molinier, D.Tanese, E. Ronzitti, Z. L. Newman, C. Wyart, E.Y. Isacoff, E. Papagiakoumou and V. Emiliani. Multiplexed temporally focused light shaping for high-resolution multi-cell targeting. Optica 5, 1478-1491 (2018).

  • Publication 2: I-Wen Chen, Eirini Papagiakoumou, and V. Emiliani. Towards circuit optogenetics, Current Opinion in Neurobiology 50, 179-189 (2018).

  • Publication 3: O. Shemesh, D. Tanese, V. Zampini, L. Changyang, P. Kiryln, E. Ronzitti, E. Papagiakoumou, E.S. Boyden, V. Emiliani, Temporally precise single-cell resolution optogenetics, Nature Neuroscience 20, 1796–1806 (2017).