All Optical

All Optical
All-optical control of neuronal circuits by optogenetics and wave front shaping

Manipulation of neuronal circuits enabling the control of each neuron independently, with single-spike precision and single-cell resolution is the next challenge to be faced in optogenetics. This means moving from whole-region optogenetics to “circuit optogenetics”. As a solution to this challenge, we propose an interdisciplinary approach combining optogenetics with wave front shaping, holographic illumination, compressed sensing and opsin engineering. We will apply this cutting-edge technology for studying neuronal circuits in optically and physiologically diverse neural systems including the mammalian cortex and the zebrafish larva.


  • (poster) I-Wen Chen, Chung Yuen Chan, Vincent de Sars, Emiliano Ronzitti, Dimitrii Tanese, Valentina Emiliani [2022]. In vivo high-throughput probing of synaptic connectivity using two-photon holographic optogenetic stimulation and compressed sensing strategies. OPTOGEN 2022.
  • I-Wen Chen, Eirini Papagiakoumou, and V. Emiliani [2018]. Towards circuit optogenetics. Current Opinion in Neurobiology 50, 179-189.
  • C. Molinier, D.Tanese, E. Ronzitti, Z. L. Newman, C. Wyart, E.Y. Isacoff, E. Papagiakoumou and V. Emiliani [2018]. Multiplexed temporally focused light shaping for high-resolution multi-cell targeting. Optica 5, 1478-1491.