All Optical

All Optical
All-optical control of neuronal circuits by optogenetics and wave front shaping

We will develop an interdisciplinary approach combining optogenetics with wave front shaping, multiphoton imaging, holographic illumination, compressed sensing and opsin engineering for precise “all-optical” large -scale control of neuronal circuits.

Manipulation of neuronal circuits enabling the control of each neuron independently, with single-spike precision and single-cell resolution is the next challenge to be faced in optogenetics. This means moving from whole-region optogenetics to “circuit optogenetics”. As a solution to this challenge, we propose an interdisciplinary approach combining optogenetics with wave front shaping, holographic illumination, compressed sensing and opsin engineering. We will apply this cutting-edge technology for studying neuronal circuits in optically and physiologically diverse neural systems including the mammalian cortex and the zebrafish larva.

Publications

  • Publication 1: C. Molinier, D.Tanese, E. Ronzitti, Z. L. Newman, C. Wyart, E.Y. Isacoff, E. Papagiakoumou and V. Emiliani. Multiplexed temporally focused light shaping for high-resolution multi-cell targeting. Optica 5, 1478-1491 (2018).

  • Publication 2: I-Wen Chen, Eirini Papagiakoumou, and V. Emiliani. Towards circuit optogenetics, Current Opinion in Neurobiology 50, 179-189 (2018).

  • Publication 3: O. Shemesh, D. Tanese, V. Zampini, L. Changyang, P. Kiryln, E. Ronzitti, E. Papagiakoumou, E.S. Boyden, V. Emiliani, Temporally precise single-cell resolution optogenetics, Nature Neuroscience 20, 1796–1806 (2017).